α-Amylase Determination Using Maltopentaose as Substrate

A novel electrochemical method to determine α-amylase activity.

In this paper, we report a novel electrochemical method that can be developed as a biosensor for simple and direct determination of α-amylase activity. The method is based on the hydrolysis of maltopentaose, the substrate of the enzyme, which is immobilized on the surface of a gold electrode, and the induced charge changes of the substrate-modified electrode. Specifically, the substrate maltope...

New chromogenic substrate for determination of serum amylase activity.

A rapid assay for serum amylase activity has been developed based on the use of a new chromogenic substrate-Cibachron Blue-amylose. The procedure requires 0.1 ml of serum and measures the production of soluble chro. mogen, the formation of which islinearwith enzyme activity. Normal human serum has a mean amylase activity of 118 ± 37 (SD) mg dye/100 ml/15 mm or, in international units, 78 ± 24 (...

Thermostable α-amylase from Lignocellulosic Residues Using Bacillus amyloliquefaciens

Evaluation of a colorimetric test for the determination of alpha-amylase with p-nitrophenylheptaoside as substrate.

A continuous method for the determination of alpha-amylase activity with a defined substrate has been published by Rauscher et al. in Berichte der Osterreichischen Gesellschaft für Klinische Chemie 4, 150 (1981). It offers the following advantages: good practicability, easy adaptation to analysers and short incubation time. Twenty-one laboratories took part in a multicentric study on this metho...

Amylase α-2A Autoantibodies

OBJECTIVE The pathogenesis of autoimmune pancreatitis (AIP) and fulminant type 1 diabetes remains unclear, although it is known that immune-mediated processes severely compromise the endocrine and exocrine functions in both diseases. RESEARCH DESIGN AND METHODS We have screened a lambdaTriplEx2 human pancreas cDNA library with serum from a patient with AIP and obtained positive clones. Sequen...